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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: CD200-CD200R dysfunction exacerbates microglial activation and dopaminergic neurodegeneration in a rat model of Parkinson’s disease | Zhang et al. Journal of Neuroinflammation 2011 8 154 http www.jneuroinflammation.eom content 8 1 154 JJOURNAL OF. NEUROINFLAMMATION RESEARCH Open Access CD200-CD200R dysfunction exacerbates microglial activation and dopaminergic neurodegeneration in a rat model of Parkinson s disease 1t 1t 1 1 1 2 1.2 Shi Zhang Xi-Jin Wang Li-Peng Tian Jing Pan Guo-Qiang Lu Ying-Jie Zhang Jian-Qing Ding and Sheng-Di Chen1 2 Abstract Background Increasing evidence suggests that microglial activation may participate in the aetiology and pathogenesis of Parkinson s disease PD . CD200-CD200R signalling has been shown to be critical for restraining microglial activation. We have previously shown that expression of CD200R in monocyte-derived macrophages induced by various stimuli is impaired in PD patients implying an intrinsic abnormality of CD200-CD200R signalling in PD brain. Thus further in vivo evidence is needed to elucidate the role of malfunction of CD200-CD200R signalling in the pathogenesis of PD. Methods 6-hydroxydopamine 6-OHDA -lesioned rats were used as an animal model of PD. CD200R-blocking antibody BAb was injected into striatum to block the engagement of CD200 and CD200R. The animals were divided into three groups which were treated with 6-OHDA Veh PBS 6-OHDA CAb isotype control antibody or 6-OHDA BAb respectively. Rotational tests and immunohistochemistry were employed to evaluate motor deficits and dopaminergic neurodegeneration in animals from each group. HPLC analysis was used to measure monoamine levels in striatum. Morphological analysis and quantification of CD11b- or MHC II- immunoreactive cells were performed to investigate microglial activation and possible neuroinflammation in the substantia nigra SN . Finally ELISA was employed to assay protein levels of proinflammatory cytokines. Results Compared with 6-OHDA CAb or 6-OHDA Veh groups rats treated with 6-OHDA BAb showed a significant increase in counts of contralateral rotation and a significant decrease in .
