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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors | Mestre et al. Journal of Neuroinflammation 2011 8 102 http www.jneuroinflammation.eom content 8 1 102 JJOURNAL1 OF. NEUROINFLAMMATION RESEARCH Open Access Anandamide inhibits Theiler s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors 1 1 1 I- I z z 1.2 1 1 Leyre Mestre Paula M Inigo Miriam Mecha Fernando G Correa Miriam Hernangomez-Herrero Frida Loria Fabian Docagne1 3 José Borrell1 and Carmen Guaza1 Abstract Background VCAM-1 represents one of the most important adhesion molecule involved in the transmigration of blood leukocytes across the blood-brain barrier BBB that is an essential step in the pathogenesis of MS. Several evidences have suggested the potential therapeutic value of cannabinoids CBs in the treatment of MS and their experimental models. However the effects of endocannabinoids on VCAM-1 regulation are poorly understood. In the present study we investigated the effects of anandamide AEA in the regulation of VCAM-1 expression induced by Theiler s virus TMEV infection of brain endothelial cells using in vitro and in vivo approaches. Methods i in vitro VCAM-1 was measured by ELISA in supernatants of brain endothelial cells infected with TMEV and subjected to AEA and or cannabinoid receptors antagonist treatment. To evaluate the functional effect of VCAM-1 modulation we developed a blood brain barrier model based on a system of astrocytes and brain endothelial cells co-culture. ii in vivo CB1 receptor deficient mice Cnr1- - infected with TMEV were treated with the AEA uptake inhibitor UCM-707 for three days. VCAM-1 expression and microglial reactivity were evaluated by immunohistochemistry. Results Anandamide-induced inhibition of VCAM-1 expression in brain endothelial cell cultures was mediated by activation of CB1 receptors. The study of leukocyte transmigration confirmed the functional relevance of VCAM-1 inhibition by AEA. In vivo approaches also .
