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Present study was conducted for standardization of processing conditions for development of biodegradable Zein films and optimization of isolated Coliphage level to be incorporated in developed film. Zein films were formulated with varying concentrations of Zein, protein and glycerol, plasticizer. Three Zein levels 5%, 6%, 7% with constant glycerol level as 7% and three glycerol levels 6.5%, 7%, 7.5% with constant Zein levels 5% were used to formulate films. Films were compared on basis of quality parameters for selection of optimum level of Zein and glycerol. Results depicted that density and film thickness increased with increase in Zein. Extensibility, penetrability, moisture decreased at higher concentration of Zein. With increase in glycerol, there was significant increase in thickness, density and extensibility but penetrability depicted only initial increase in films. On basis of above findings, 5% Zein and 7% glycerol was selected for development of bioactive film. Coliphages incorporated in Zein films were isolated by agar overlay technique. % of buffer solution containing coliphages to be incorporated was selected on basis of diameter of inhibition halos. Storage stability of Zein films were evaluated for 4 weeks at 25±2○C. | Quality and storage stability of bioactive biodegradable zein protein films impregnated with Coliphages
