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Using various chromatographic experiments, four sterols as 5α,8α-epidioxy-24(S)-ethylcholest-6-en-3β-ol (1), (22E,24S)-5α,8α-epidioxy-24-ethyl-cholesta-6,22-dien-3β-ol (2), 7-oxocholesterol (3), and (22E)-3β-hydroxycholesta5,22-dien-7-one (4) were isolated from the methanol extract of the blood cockle Anadara granosa. The structures of isolated compounds were elucidated by 1D and 2D-NMR experiments in comparison with reported data. This is the first report of these compounds from A. granosa. | Vietnam Journal of Chemistry, International Edition, 54(6): 786-789, 2016 DOI: 10.15625/0866-7144.2016-00405 Steroids from the blood cockle Anadara granosa Phan Thi Thanh Huong1, Nguyen Hoang Giang2, Tran Thi Hong Hanh1, Do Cong Thung3, Nguyen Xuan Cuong1, Nguyen Van Thanh1, Chau Van Minh1* 1 Institute of Marine Biochemistry, Vietnam Academy of Science and Technology 2 3 Faculty of Chemistry, VNU University of Science Institute of Marine Environment and Resources, VAST Received 24 November 2016; Accepted for publication 19 December 2016 Abstract Using various chromatographic experiments, four sterols as 5α,8α-epidioxy-24(S)-ethylcholest-6-en-3β-ol (1), (22E,24S)-5α,8α-epidioxy-24-ethyl-cholesta-6,22-dien-3β-ol (2), 7-oxocholesterol (3), and (22E)-3β-hydroxycholesta5,22-dien-7-one (4) were isolated from the methanol extract of the blood cockle Anadara granosa. The structures of isolated compounds were elucidated by 1D and 2D-NMR experiments in comparison with reported data. This is the first report of these compounds from A. granosa. Keywords. Anadara granosa, Arcidae, blood cockle, steroid. 1. INTRODUCTION With more than 100,000 species, the phylum Mollusca is the second largest after the Arthropoda and, like the latter, the Mollusca includes as many marine species as terrestrial species. Of these, mollusk shells have been of considerable economic importance since antiquity [1]. The blood cockle Anadara granosa is one important sea product in Vietnam and some Asian countries. As a part of our investigations on chemical constituents of Vietnamese mollusks, we report herein the isolation and structure identification of four steroids from A. granosa. (Supelco, USA) resins. For thin layer chromatography (TLC) there used pre-coated silica gel 60 F254 (1.05554.0001, Merck) and RP-18 F254S plates (1.15685.0001, Merck). Compounds were visualized by spraying with aqueous 10 % H2SO4 and heating for 3-5 minutes. 2.2. Marine materials The samples of blood cockle A. .
