Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: "Effect of prolonging radiation delivery time on retention of gammaH2AX. | Radiation Oncology BioMed Central Open Access Short report Effect of prolonging radiation delivery time on retention of gammaH2AX Vitali Moiseenko1 Judit P Banáth2 Cheryl Duzenli1 and Peggy L Olive 2 Address 1Medical Physics Department British Columbia Cancer Agency Vancouver Canada and 2Medical Biophysics Department British Columbia Cancer Research Centre Vancouver Canada Email Vitali Moiseenko - vmoiseenko@bccancer.bc.ca Judit P Banáth - jbanath@bccrc.ca Cheryl Duzenli - cduzenli@bccancer.bc.ca Peggy L Olive - polive@bccrc.ca Corresponding author Published 27 June 2008 Received 20 February 2008 Radiation Oncology 2008 3 18 doi 10.1186 1748-717X-3-18 Accepted 27 June 2008 This article is available from http www.ro-journal.cOm content 3 1 18 2008 Moiseenko et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background and purpose Compared to conventional external beam radiotherapy IMRT requires significantly more time to deliver the dose. Prolonging dose delivery potentially increases DNA repair which would reduce the biological effect. We questioned whether retention of yH2AX a measure of lack of repair of DNA damage would decrease when dose delivery was protracted. Materials and methods Exponentially growing SiHa cervical carinoma cells were irradiated with 6 MV photons in a water tank using a VarianEX linear accelerator. Cells held at 37 C received 2 Gy in 0.5 min and 4 Gy in I min. To evaluate effect of dose delivery prolongation 2 and 4 Gy were delivered in 30 and 60 min. After 24 h recovery cells were analyzed for clonogenic survival and for residual yH2AX as measured using flow cytometry. Results Increasing the dose delivery time from 0.5 or 1 min to 30 or 60 min produced a signficant increase in cell .
