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Trichoderma reesei is a widely distributed soil fungus that antagonizes numerous fungal phytopathogens. Random induced mutation by gamma radiation with five treatments TrME 1 (50 Kr), TrME 2 (100 Kr), TrME 3 (150 Kr), TrME 4 (200 Kr), TrME 5 (untreated) were studied for morphological study and chitinase activity. The result indicates that TrME 1 contain maximum i.e. 0.3201 chitinase enzyme units/mg of protein followed by TrME 4 recording 0.3108 chitinase enzyme units/mg of protein. This indicates that treatments TrME 1 and TrME 4 are the best mutants for use as effective mycoparasite in the biocontrol of plant pathogenic fungi. |
