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A study on the development of in vitro propagation protocol for newly developed mutants of Carnation (Dianthus caryophyllus L.)” was carried out during 2017-2018. Results reveal that all the mutants responded well to the in vitro propagation, however ‘UHFS Car-4’ exhibited best propagation potential. Calcium Hypochlorite (5%) treatment for 20 min. is suggested for surface sterilization of explants as it gives 100% uncontaminated growing cultures. For initial culture establishment, MS medium supplemented with 2.0 mgl-1 BA proved to be most effective resulting in earliest establishment. Shoot tips were found better than nodal segment explants for multiplication of carnation. | Development of in vitro propagation protocol for newly developed mutants of carnation (Dianthus caryophyllus L.)