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Evaluation of antioxidant and antimicrobial activity of seaweed (Sargassum sp.) extract: A study on inhibition of glutathione-S-transferase activity

In the present study, the free radical scavenging potentials (DPPH radical and hydroxyl radical), inhibition of lipid peroxidation, and glutathione-S-transferase and antimicrobial properties of Sargassum sp. extract were investigated. The tested extract exhibited a dose-dependent free radical scavenging action against DPPH radical and hydroxyl radical and antimicrobial activity | Turk J Biol 32 (2008) 119-125 © TÜB‹TAK Evaluation of Antioxidant and Antimicrobial Activity of Seaweed (Sargassum sp.) Extract: A Study on Inhibition of Glutathione-S-Transferase Activity Jayanta Kumar PATRA1, Sakti Kanta RATH1, Karmabeer JENA2, Vijaya Kumar RATHOD2, Hrudayanath THATOI1 1 P. G. Department of Biotechnology, North Orissa University, Baripada-757 003,Orissa - INDIA 2 Biological Oceanography Division, National Institute of Oceanography, Dona Paula, Goa- 403 004 - INDIA Received: 06.07.2007 Abstract: In the present study, the free radical scavenging potentials (DPPH radical and hydroxyl radical), inhibition of lipid peroxidation, and glutathione-S-transferase and antimicrobial properties of Sargassum sp. extract were investigated. The tested extract exhibited a dose-dependent free radical scavenging action against DPPH radical and hydroxyl radical and antimicrobial activity. In addition, inhibition of lipid peroxidation and glutathione-S-transferase activities were also observed. The overall results have established that this seaweed could be used against several diseases and, in the food processing industry, to preserve foods. Key Words: Antimicrobial activity, free radical, glutathione-S-transferase, hydroxyl radical, lipid peroxidation Deniz yosunu (Sargassum sp.) Ekstresinin Antioksidant ve Antimikrobial Aktivitesinin ‹ncelenmesi: Glutatyon-S-transferaz Aktivitesinin ‹nhibisyonu Özet: Sargassum sp. ekstrelerinin radikal süpürücü aktivitesi (DPPH ve hidroksil radikali), lipid peroksidasyon inhibisyonu, ve antimikrobiyal aktivitesi incelenmifltir. Buna ilave olarak lipid peroksidasyonu ve glutatyon-S-transferaz aktivitesi de araflt›r›lm›flt›r. Sonuç olarak, deniz yosununun baz› hastal›klara iyi gelebilece¤i ve besinlerin saklanmas›nda kullan›labilece¤i ortaya ç›kar›lm›flt›r. Anahtar Sözcükler: Antimikrobiyal aktivite, serbest radikal, glutatyon-S-transferaz hidroksil radikal, lipid peroksidasyonu Introduction Reactive oxygen species (ROS) (e.g.,