Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
The core structure of the lipopolysaccharide (LPS) isolated from a rough strain of the phytopathogenic bacterium Pseudomonas syringaepv.phaseolicola, GSPB 711, was investigated by sugar and methylation analyses, Fourier transform ion-cyclotron resonance ESI MS, and one- and two-dimensional 1 H-, 13 C- and 31 P-NMR spectroscopy. Strong alkaline deacylation of the LPS resulted in two core-lipid A backbone undecasaccharide pentakisphos-phates in the ratio 2.5 : 1, which corresponded to outer core glycoforms 1 and 2 terminated with either L-rham-nose or 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), res-pectively | Eur. J. Biochem. 271 4968-4977 2004 FEBS 2004 doi 10.1111 j.1432-1033.2004.04467.x Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola Evelina L. Zdorovenko1 2 Evgeny Vinogradov Galina M. Zdorovenko3 Buko Lindner2 Olga V. Bystrova1 2 Alexander S. Shashkov1 Klaus Rudolph4 Ulrich Zahringer2 and Yuriy A. Knirel1 2 1N. D. Zelinsky Institute of Organic Chemistry Russian Academy of Sciences Moscow Russia 2Research Center Borstel Leibniz Center for Medicine and Biosciences Borstel Germany 3D.K. Zabolotny Institute of Microbiology and Virology National Academy of Sciences of Ukraine Kiev Ukraine 4Institute for Plant Pathology and Plant Defence Georg August University Gottingen Germany The core structure of the lipopolysaccharide LPS isolated from a rough strain of the phytopathogenic bacterium Pseudomonas syringae pv. phaseolicola GSPB 711 was investigated by sugar and methylation analyses Fourier transform ion-cyclotron resonance ESI MS and one- and two-dimensional 1H- 13C- and 31P-NMR spectroscopy. Strong alkaline deacylation of the LPS resulted in two core-lipid A backbone undecasaccharide pentakisphos-phates in the ratio w 2.5 1 which corresponded to outer core glycoforms 1 and 2 terminated with either L-rham-nose or 3-deoxy-D-manno-oct-2-ulosonic acid Kdo respectively. Mild acid degradation of the LPS gave the major glycoform 1 core octasaccharide and a minor truncated glycoform 2 core heptasaccharide which resulted from the cleavage of the terminal Kdo residues. The inner core of P. syringae is distinguished by a high degree of phosphorylation of L-glycero-D-manno-heptose residues with phosphate diphosphate and ethanolamine diphosphate groups. The glycoform 1 core is structurally similar but not identical to one of the core glycoforms of the human pathogenic bacterium Pseudomonas aeruginosa. The outer core composition and structure may be useful as a chemotaxonomic marker for the P. syringae group of .
