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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Trichoderma viride cellulase induces resistance to the antibiotic pore-forming peptide alamethicin associated with changes in the plasma membrane lipid composition of tobacco BY-2 cells | Aidemark et al. BMC Plant Biology 2010 10 274 http www.biomedcentral.com 1471-2229 10 274 BMC Plant Biology RESEARCH ARTICLE Open Access Trichoderma viride cellulase induces resistance to the antibiotic pore-forming peptide alamethicin associated with changes in the plasma membrane lipid composition of tobacco BY-2 cells h I r I Al z l Z m r lz1 I_I Z rì I I I Z I I rzA ZZI2 3 A -s C 1 -s c zd Z I I I I r 3 I_I Z z I lz Ct 11 z -s zd4 c r I lz A zd rr z 5 Marl AidemarK nenriK Ijellstrom Anna Stina Sandelius renriK Stalbrand triK Andreasson Allan G Rasmusson1 Susanne Widell1 Abstract Background Alamethicin is a membrane-active peptide isolated from the beneficial root-colonising fungus Trichoderma viride. This peptide can insert into membranes to form voltage-dependent pores. We have previously shown that alamethicin efficiently permeabilises the plasma membrane mitochondria and plastids of cultured plant cells. In the present investigation tobacco cells Nicotiana tabacum L. cv Bright Yellow-2 were pre-treated with elicitors of defence responses to study whether this would affect permeabilisation. Results Oxygen consumption experiments showed that added cellulase already upon a limited cell wall digestion induced a cellular resistance to alamethicin permeabilisation. This effect could not be elicited by xylanase or bacterial elicitors such as flg22 or elf18. The induction of alamethicin resistance was independent of novel protein synthesis. Also the permeabilisation was unaffected by the membrane-depolarising agent FCCP. As judged by lipid analyses isolated plasma membranes from cellulase-pretreated tobacco cells contained less negatively charged phospholipids PS and PI yet higher ratios of membrane lipid fatty acid to sterol and to protein as compared to control membranes. Conclusion We suggest that altered membrane lipid composition as induced by cellulase activity may render the cells resistant to alamethicin. This induced resistance could reflect a natural .
