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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Mouse DNA contamination in human tissue tested for XMRV. | Robinson et al. Retrovirology 2010 7 108 http www.retrovirology.eom content 7 1 108 RETROVIROLOGY RESEARCH Open Access Mouse DNA contamination in human tissue tested for XMRV 11 11 1 1 2 3 Mark J Robinson Otto W Erlwein Steve Kaye Jonathan Weber Oya Cingoz Anup Patel Marjorie M Walker4 Wun-Jae Kim5 Mongkol Uiprasertkul6 John M Coffin2 Myra O McClure 1 Abstract Background We used a PCR-based approach to study the prevalence of genetic sequences related to a gammaretrovirus xenotropic murine leukemia virus-related virus XMRV in human prostate cancer. This virus has been identified in the US in prostate cancer patients and in those with chronic fatigue syndrome. However with the exception of two patients in Germany XMRV has not been identified in prostate cancer tissue in Europe. Most putative associations of new or old human retroviruses with diseases have turned out to be due to contamination. We have looked for XMRV sequences in DNA extracted from formalin-fixed paraffin- embedded prostate tissues. To control for contamination PCR assays to detect either mouse mitochondrial DNA mtDNA or intracisternal A particle IAP long terminal repeat DNA were run on all samples owing to their very high copy number in mouse cells. Results In general agreement with the US prevalence XMRV-like sequences were found in 4.8 of prostate cancers. However these were also positive as were 21.5 of XMRV-negative cases for IAP sequences and many but not all were positive for mtDNA sequences. Conclusions These results show that contamination with mouse DNA is widespread and detectable by the highly sensitive IAP assay but not always with less sensitive assays such as murine mtDNA PCR. This study highlights the ubiquitous presence of mouse DNA in laboratory specimens and offers a means of rigorous validation for future studies of murine retroviruses in human disease. Background In 2006 XMRV was identified in stromal cells associated with prostate cancers of men with a family history of the .
