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Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Assessing mechanical integrity of spinal fusion by in situ endochondral osteoinduction in the murine model | Dewan et al. Journal of Orthopaedic Surgery and Research 2010 5 58 http www.josr-online.eom content 5 1 58 JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH RESEARCH ARTICLE Open Access Assessing mechanical integrity of spinal fusion by in situ endochondral osteoinduction in the murine model 1 1 1 1 22 Ashvin K Dewan Rahul A Dewan Nathan Calderon Angie Fuentes ZaWaunyka Lazard Alan R Davis Michael Heggeness1 John A Hipp1 Elizabeth A Olmsted-Davis2 Abstract Background Historically radiographs micro-computed tomography micro-CT exams palpation and histology have been used to assess fusions in a mouse spine. The objective of this study was to develop a faster cheaper reproducible test to directly quantify the mechanical integrity of spinal fusions in mice. Methods Fusions were induced in ten mice spine using a previously described technique of in situ endochondral ossification harvested with soft tissue and cast in radiolucent alginate material for handling. Using a validated software package and a customized mechanical apparatus that flexed and extended the spinal column the amount of intervertebral motion between adjacent vertebral discs was determined with static flexed and extended lateral spine radiographs. Micro-CT images of the same were also blindly reviewed for fusion. Results Mean intervertebral motion between control non-fused spinal vertebral discs was 6.1 0.2 during spine flexion extension. In fusion samples adjacent vertebrae with less than 3.5 intervertebral motion had fusions documented by micro-CT inspection. Conclusions Measuring the amount of intervertebral rotation between vertebrae during spine flexion extension is a relatively simple cheap 100 clinically relevant and fast test for assessing the mechanical success of spinal fusion in mice that compared favorably to the standard micro-CT. Background Spinal fusion is a common surgical procedure used to manage a variety of disorders. In 2001 over 50 of all inpatient lumbar spine operations other than those
