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Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro | BioMed Central Virology Journal Research Open Access Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro David Franco1 Harsh B Pathak2 Craig E Cameron2 Bart Rombaut3 Eckard Wimmer1 and Aniko V Paul 1 Address Department of Molecular Genetics and Microbiology School of Medicine Stony Brook University Stony Brook N. Y. 11790 USA 2Department of Biochemistry and Molecular Biology Pennsylvania State University University Park Pennsylvania 16802 USA and 3Department of Microbiology and Hygiene Vrije Universiteit Brussel B-1090 Brussels Belgium Email David Franco - davidfranco72@yahoo.com Harsh B Pathak - hxp141@psu.edu Craig E Cameron - cec9@psu.edu Bart Rombaut - brombaut@vub.ac.be Eckard Wimmer - ewimmer @ms.cc.sunysb.edu Aniko V Paul - apaul@notes.cc.sunysb.edu Corresponding author Published 21 November 2005 Received 30 June 2005 Accepted 21 November 2005 Virology Journal 2005 2 86 doi l0.ll86 l743-422X-2-86 This article is available from http www.virologyj.com content 2 1 86 2005 Franco et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Poliovirus protein 3CDpro possesses both proteinase and RNA binding activities which are located in the 3Cpro domain of the protein. The RNA polymerase 3Dpo1 domain of 3CDpro modulates these activities of the protein. We have recently shown that the level of 3CDpro in HeLa cell-free in vitro translation-RNA replication reactions is suboptimal for efficient virus production. However the addition of either 3CDpro mRNA or of purified 3CDpro protein to in vitro reactions programmed with viral RNA results in a 100-fold increase in virus yield. Mutational analyses of 3CDpro indicated that RNA .
