Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells | J. Vet. Sci. 2009 10 1 23-28 DOI 10.4142 jvs.2009.10.1.23 JOURNAL OF Veterinary Science Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells 1 1 2 1 1 _1 1 Hyun Sun Cho Seung Hee Chang Youn Sun Chung Ji Young Shin Sung Jin Park Eun Sun Lee Soon Kyung Hwang1 Jung Taek Kwon1 Arash Minai Tehrani1 Minah Woo1 Mi Sook Noh1 Huda Hanifah1 Hua Jin1 Cheng Xiong Xu1 Myung Haing Cho1 2 laboratory of Toxicology College of Veterinary Medicine and 2Nano Systems Institute-National Core Research Center Seoul National University Seoul 151-742 Korea Tetrandrine TET a bis-benzylisoquinoline alkaloid from the root of Stephania tetrandra is known to have anti-tumor activity in various malignant neoplasms. However the precise mechanism by which TET inhibits tumor cell growth remains to be elucidated. The present studies were performed to characterize the potential effects of TET on phosphoinositide 3-kinase Akt and extracellular signal-regulated kinase ERK pathways since these signaling pathways are known to be responsible for cell growth and survival. TET suppressed cell proliferation and induced apoptosis in A549 human lung carcinoma cells. TET treatment resulted in a down-regulation of Akt and ERK phosphorylation in both time- concentration-dependent manners. The inhibition of ERK using PD98059 synergistically enhanced the TET-induced apoptosis of A549 cells whereas the inhibition of Akt using LY294002 had a less significant effect. Taken together our results suggest that TET i selectively inhibits the proliferation of lung cancer cells by blocking Akt activation and ii increases apoptosis by inhibiting ERK. The treatment of lung cancers with TET may enhance the efficacy of chemotherapy and radiotherapy and increase the apoptotic potential of lung cancer cells. Keywords A549 cells Akt apoptosis Erk tetrandrine Introduction Apoptosis also called programmed cell death is essential for the homeostasis of normal tissues. Altering the .
