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Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection | J. Vet. Set. 2005 6 4 349-352 JOURNAL OF Short Communication Veterinary Science Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection Jin Sik Oh Dae Sub Song Jeong Sun Yang Ju Young Song Hyoung Joon Moon Tae Yung Kim Bong Kyun Park Department of Veterinary Medicine College of Veterinary Medicine and School of Agricultural Biotechnology Seoul National University Seoul 151-742 Korea An indứect porcine epidemic diarrhea PED virus PEDV enzyme-linked immunosorbent assay ELISA was compared with the serum neutralization SN test by testing 46 samples from experimentally infected sows 73 samples from naive sows and 1 024 field sow samples from 48 commercial swine farms of undefined PED status. The SN test and the ELISA were performed using PEDV KPEDV-9 strain. Viral proteins as a coating antigen of PEDV ELISA were extracted from the cytoplasm of PEDV-infected Vero cells using a non-ionic detergent Triton X-100 and a simple protocol of PEDV ELISA was followed. The presence of antibodies in these experimental samples was confirmed by SN and ELISA in which the sensitivity of the ELISA was 89.1 and the corresponding specificity was 94.5 . On testing 1 024 field samples an overall agreement of 84.2 was generated between the SN and ELISA. This study demonstrates that the PEDV ELISA is a useful serodiagnostic screening test at herd level for detecting swine antibodies against PEDV. Key words ELISA porcine epidemic diarrhea virus serum neutralization test Porcine epidemic diarrhea virus PEDV a member of the coronavirus group I 17 causes watery diarrhea dehydration and high mortality in suckling pigs through the destruction of epithelial cells of the small intestine 4 14 A serological diagnosis for porcine epidemic diarrhea PED has been made through the demonstration of antibodies against PEDV. Currently the serum neutralization SN test is the serological method used most commonly in .
