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Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Induction of castration by immunization of male dogs with recombinant gonadotropin-releasing hormone (GnRH)-canine distemper virus (CDV) T helper cell epitope p35 | J. Vet. Sci. 2005 6 1 21-24 JOURNAL OF Veterinary Science Induction of castration by immunization of male dogs with recombinant gonadotropin-releasing hormone GnRH -canine distemper virus CDV T helper cell epitope p35 Mi-Jeong Jung1 Young-Chan Moon1 Ik-Hyun Cho2 Jung-Yong Yeh1 Sun-Eui Kim1 Wha-Seok Chang1 Seung-Young Park1 Chang-Seon Song1 Hwi-Yool Kim3 Keun-Kyu Park1 Steven McOrist4 In-Soo Choi1 Joong-Bok Lee1 Department of Infectious Diseases College of Veterinary Medicine Konkuk University Seoul 143-701 Korea Department of Physiology College of Dentistry Seoul National University Seoul 110-749 Korea Department of Veterinary Surgery College of Veterinary Medicine Konkuk University Seoul 143-701 Korea 4Tufts University School of Veterinary Medicine Westboro Road North Grafton Massachusetts 01536 USA Immunocastration is a considerable alternative to a surgical castration method especially in male animal species for alleviating unwanted male behaviors and characteristics. Induction of high titer of antibody specific for gonadotropin-releasing hormone GnRH correlates with the regression of testes. Fusion proteins composed of canine GnRH and T helper Th cell epitope p35 originated from canine distemper virus CDV F protein and goat rotavirus VP6 protein were produced in E. coli. When these fusion proteins were injected to male dogs which were previously immunized with CDV vaccine the fusion protein of GnRH-CDV Th cell epitope p35 induced much higher antibody than that of GnRH-rotavirus VP6 protein or GnRH alone. The degeneration of spermatogenesis was also verified in the male dogs immunized with the fusion protein of GnRH-CDV Th cell epitope p35. These results indicate that canine GnRH conjugated to CDV Th cell epitope p35 acted as a strong immunogen and the antibody to GnRH specifically neutralized GnRH in the testes. This study also implies a potential application of GnRH-based vaccines for immunocastration of male pets. Key words Immunocastration GnRH canine .
