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Microorganisms associated with the production of volatile compound in spoilt mango fruits sold in Sokoto town were isolated and identified. The organisms include seven species of bacteria and a species of yeast. These include Bacillus pumilus, Bacillus firmus, Brevibacillus laterosporus, Morganella morganii, Paenibacillus alvei, Staphylococcus saccharolyticus, Listeria monocytogenes and Candida krusei respectively. GC-MS analysis revealed the presence of eleven and sixteen volatile organic compound in the healthy and spoilt ripe mango fruits. Octadecanoic acid, oleic acid, 1 – Butanol, 3 – methyl-, carbonate (2:1) and 3,7 – Dimethyl nonane were common to both healthy and spoilt fruits with the first. | APPENDIX A TO PART 136 METHODS FOR ORGANIC CHEMICAL ANALYSIS OF MUNICIPAL AND INDUSTRIAL WASTEWATER METHOD 610 POLYNUCLEAR AROMATIC HYDROCARBONS 1. Scope and Application 1.1 This method covers the determination of certain polynuclear aromatic hydrocarbons PAH . The following parameters can be determined by this method Parameter STORET No. CAS No. Acenaphthene 34205 83-32-9 Acenaphthylene 34200 208-96-8 Anthracene 34220 120-12-7 Benzo a anthracene 34526 56-55-3 Benzo a pyrene 34247 50-32-8 Benzo b fluoranthene 34230 205-99-2 Benzo ghi perylene 34521 191-24-2 Benzo k fluoranthene 34242 207-08-9 Chrysene 34320 218-01-9 Dibenzo a h anthracene 34556 53-70-3 Fluoranthene 34376 206-44-0 Fluorene 34381 86-73-7 Indeno 1 2 3-cd pyrene 34403 193-39-5 Naphthalene 34696 91-20-3 Phenanthrene 34461 85-01-8 Pyrene 34469 129-00-0 1.2 This is a chromatographic method applicable to the determination of the compounds listed above in municipal and industrial discharges as provided under 40 CFR Part 136.1. When this method is used to analyze unfamiliar samples for any or all of the compounds above compound identifications should be supported by at least one additional qualitative technique. Method 625 provides gas chromatograph mass spectrometer GC MS conditions appropriate for the qualitative and quantitative confirmation of results for many of the parameters listed above using the extract produced by this method. 1.3 This method provides for both high performance liquid chromatographic HPLC and gas chromatographic GC approaches for the determination of PAHs. The gas chromatographic procedure does not adequately resolve the following four pairs of compounds Anthracene and phenanthrene chrysene and benzo a anthracene benzo b fluoranthene and benzo k fluoranthene and dibenzo a h anthracene and indeno 1 2 3-cd pyrene. Unless the purpose for the analysis can be served by reporting the sum of an unresolved pair the liquid chromatographic approach must be used for these compounds. The liquid
