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Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine | J. Vet. Set. 2006 7 3 271-275 JOURNAL OF Veterinary Science Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine Dong-Kun Yang Byoung-Han Kim Seong-In Lim Jun-Hun Kwon Kyung-Woo Lee Cheong-Up Choi Chang-Hee Kweon National Veterinary Research and Quarantine Service Ministry of Agriculture and Forestry Anyang 430-824 Korea The Japanese encephalitis virus JEV is one of causative agents of reproductive failure in pregnant sows. An indirect enzyme-linked immunosorbent assay I-ELISA was examined for its potential use in the rapid monitoring of the JEV and the results were compared with those from the hemagglutination inhibition HI and serum neutralization SN tests. The comparative analysis showed that the results of I-ELISA showed a significant correlation with the conventional HI r 0.867 and SN tests r 0.804 respectively. When the I-ELISA results were compared with the traditional diagnostic assays the sensitivity of the I-ELISA was 94.3 with the HI test and 93.7 with the SN test respectively. The specificity was found to be 81.4 and 80.0 with the HI and SN tests respectively. To determine the applicability of I-ELISA in the field the serum samples from 720 pigs were collected from 4 regions in Korea between July and August 2004. The results indicated that 21.7 of screened pigs were seropositive for the JEV. The seropositive rates of JEV in the 4 provinces were 12.6 in Gyeonggi 45.0 in Gyeongnam 16.7 in Jeonbuk and 12.2 in Jeju. The I-ELISA methodology developed in this study was shown to have considerable sensitivity and specificity through a comparison with HI and the SN tests. Therefore it might be one of convenient methods for screening a large number of samples in various fields. Key words diagnostic efficiency I-ELISA Japanese encephalitis virus JEV Introduction The Japanese encephalitis virus JEV is a small enveloped virus with a single stranded positive sense RNA genome approximately 11 kb long
