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Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains | J. Vet. Sci. 2006 7 3 263-270 JOURNAL OF Veterinary Science Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains Sri Hartati Asmarani Kusumawati Hastari Wuryastuti J. Sri Widada department of Internal Medicine and2Department of Reproduction Faculty of Veterinary Medicine and3Study Center of Biotechnology Gadjah Mada University Yogyakarta 55281 Indonesia Pathogens and Immunity UMR5119 CNRS Université Montpellier II 34095 Montpellier Cedex 5 France Toxoplasma gondii is a persistent protozoan parasite capable of infecting almost any warm-blooded vertebrates. SAG1 p30 is the prototypic member of a superfamily of surface antigens called SRS SAGl-related sequence . It constitutes the most abundant and predominant antigen. In this paper the primary structure of mature SAG1 gene of an Indonesian T. gondii isolate is described and sequence comparison is made with published sequence data of 7 other strains or isolates. Sequence comparison indicated that SAG1 is highly conserved through evolution and despite parasite spreading world-wide. Sequences may be divided into two major families independent of the strain isolate geographic origin. Variations were mainly localized at the C-terminal half or domain 2 and some clustered in restricted areas. Sequence comparison allowed US to define the Indonesian isolate as genuine virulent RH strain. A phylogenetic tree of Toxoplasma strains isolates was constructed based on SAG1. Key words Indonesian isolate p30 RH strain SAG1 sequence comparison Toxoplasma gondii Introduction Toxoplasmosis is a widely prevalent zoonosis in humans and wann-blooded animals world-wide due to the tissue cyst-forming coccidium Toxoplasma gondii. T. gondii is an obligate intracellular parasite which belongs to the phylum Ảpicomplexa a large group of mostly intracellular parasites that includes some deadly pathogens of humans and livestock. While toxoplasmosis is usually innocuous or asymptomatic in most .
