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Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Characterization of Chinook head salmon embryo phenotypes of infectious salmon anemia virus by real-time RT-PCR | J. Vet. Set. 2006 7 2 177-180 JOURNAL OF Veterinary Science Evaluation of a Bacillus stearothermophilus tube test as a screening tool for anticoccidial residues in poultry Anakalo Shitandi Aila Oketch Symon Mahungu GuidfordInstitute Microbiology Laboratory Egerton University Njoro Njoro 20107 Kenya A Bacillus stearothermophilus var. calidolactis C953 tube test was evaluated for its ability in detecting the residue of selected anticoccidial drugs in poultry specically sulfamethazine furazolidone and amprolium. Various concentrations of each drug were injected into chicken liver and kidney tissues and these tissues were tested to determine the drug detection limits for each drug. The detection limit was defined as the drug concentration at which 95 of the test results were interpreted as positive. The limits of detection in liver tissue were 0.35 pg ml for furazolidone 0.70 pg ml for sulfamethazine and 7.80 pg ml for amprolium. In kidney tissues they were 0.30 pg ml for furazolidone 0.54 pg ml for sulfamethazine and 7.6 pg ml for amprolium. It was concluded that this tube test could be used to screen for the residue of these three drugs in poultry. Key words anticoccidial drug Bacillus stearothermophilus detection limit poultry tissues Introduction The production of broiler meat has been on the rise in Kenya and represents an increased opportunity to generate income. Its rise in popularity is based on the availability of a ready market for such meat low capital requứement minimal space requứement proximity to hatcheries and the availability of a wide selection of animal feeds in urban areas 6 . Production is affected however by various diseases of which coccidiosis is particularly important 3 4 Coccidiostats are widely used to prevent this condition to find animals that have the disease and need treatment and to improve feed conversion efficiency and the rate of animal growth 1 8 . Harmful coccidial residues may appear in foods because of the extensive use of age of
