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Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells | J. Vet. Sci. 2004 5 1 11-18 JOURNAL OF Veterinary Science Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells Jin-Hyuk Choi Jun-Sung Kim Young-Chul Kim1 Yoon-Shin Kim2 Nam-Hyun Chung3 and Myung-Haing Cho Laboratory of Toxicology College of Veterinary Medicine and School of Agricultural Biotechnology Seoul National University Seoul 151-742 Korea 1 Department of Public Health College of Natural Science Keimyung University Taegu 704-701 Korea 1Institute of Environmental andIndustialMedicine Hanyang University Seoul 133-791 Korea College of Life and Environmental Sciences Korea University Seoul 136-704 Korea Accurate estimation of the exposure-response relationship between ambient urban particulate matters PM and public health is important for regulatory perspective of ambient urban particulate matters PM . Ambient PM contains various transition metals and organic compounds. PM10 aerodynamic diameter less than 10 pm is known to induce diverse diseases such as chronic cough bronchitis chest illness etc. However recent evaluation of PM2.5 aerodynamic diameter less than 2.5 pm against health outcomes has suggested that the fine particles may be more closely associated with adverse respiratory health effects than particles of larger size. This study was performed to evaluate PM2.5-induced oxidative stress in rat lung epithelial cell in order to provide basic data for the risk assessment of PM2.5. PM2.5 showed higher cytotoxicity than PM10. Also PM 2.5 induced more malondialdehyde MDA formation than PM10. In Hoechst 33258 dye staining and DNA fragmentation assay apopotic changes were clearly detected in PM2.5 treated cells in compared to PM10. Expression of catalase mRNA was increased by PM2.5 rather than PM10. PM2.5 induced higher Mth1 mRNA than PM10. In pBR322 DNA treated with PM2.5 production of single strand breakage of DNA was higher than that of PM10. In Western blot analysis PM2.5 induced more Nrf-2 protein associated with .
