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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Evaluation of an immunochromatographic assay for the detection of anti-hepatitis A virus IgM | Lee et al. Virology Journal 2010 7 164 http www.virologyj.eom content 7 1 164 VIROLOGY JOURNAL RESEARCH Open Access Evaluation of an immunochromatographic assay for the detection of anti-hepatitis A virus IgM 1 1 2 2 2 3 3 Hyeok-Jin Lee Hye Sook Jeong Byung-Ki Cho Mi-Jeong Ji Ji-Ha Kim An-Na Lee Kyoung-Ryul Lee Doo-Sung Cheon 1 Abstract Background Hepatitis A virus HAV is a causative agent of acute hepatitis which is transmitted by person-to-person contact and via the faecal-oral route. Acute HAV infection is usually confirmed by anti-HAV IgM detection. In order to detect anti-HAV IgM in the serum of patients infected with HAV we developed a rapid assay based on immunochromatography ICA and evaluated the sensitivity of this assay by comparing it with a commercial microparticle enzyme immunoassay MEIA that is widely used for serological diagnosis. Results The newly developed ICA showed 100 sensitivity and specificity when used to test 150 anti-HAV IgM-positive sera collected from infected patients and 75 negative sera from healthy subjects. Also the sensitivity of ICA is about 10 times higher than MEIA used in this study by determining end point to detect independent on infected genotype of HAV. In addition the ICA was able to detect 1 positive sample from among 50 sera from acute hepatitis patients that had tested negative for anti-HAV IgM using the MEIA. Conclusion Conclusively ICA for the detection of anti-HAV IgM will be very effective for rapid assay to apply clinical diagnosis and epidemiological investigation on epidemics due to the simplicity rapidity and specificity. Background Hepatitis A virus HAV is one of the common causative agents of acute hepatitis worldwide 1 . The clinical manifestation of HAV infection in humans can vary greatly ranging from asymptomatic infection to fulminant hepatitis 2 3 . As a result of improvements in public sanitation and hygiene conditions there has been a striking reduction in HAV endemicity in Western countries over the .
