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Tham khảo tài liệu 'intelligent and biosensors 2012 part 9', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Mercaptobenzothiazole-on-Gold Organic Phase Biosensor Systems 3. Thick-Film Biosensors for Organophosphate and Carbamate Pesticide Determination 191 was added to the Au MBT PANI AChE PVAc biosensor Albareda-Sirvent et al. 2001 Pritchard et al. 2004 Bucur et al. 2005 Somerset et al. 2009 . 2.11 Long-term stability investigation of Au MBT PANI AChE biosensor The operation of the Au MBT PANI AChE PVAc biosensor was evaluated at different time intervals of 7 day periods for a total of 30 days using one specific biosensor. A 1 ml test solution containing 0.1 M phosphate buffer 0.1 M KCl solution was degassed with argon before any substrate was added. The Au MBT PANI AChE PVAc biosensor was then evaluated in the 1 ml test solution with small aliquots of the substrate consisting of 0.01 M acetylthiocholine ATCh being added to the test solution followed by degassing. The maximum current response of the biosensor was then obtained after 2 mM of the ATCh substrate was added to the Au MBT PANI AChE PVAc biosensor. This procedure was performed on 0 7 14 21 and 28 days using one specific Au MBT PANI AChE PVAc biosensor Albareda-Sirvent et al. 2001 Somerset et al. 2009 . 2.12 Temperature stability investigation of Au MBT PANI AChE biosensor The temperature stability of the Au MBT PANI AChE PVAc biosensor was evaluated at different temperature values. To achieve this the optimum temperature for AChE activity in the constructed biosensor was determined by assaying the biosensor at various temperatures of 10 15 20 25 30 and 35 C. A 1 ml test solution containing 0.1 M phosphate buffer 0.1 M KCl solution was degassed with argon before any substrate was added and incubated in a small water bath for approximately 10 minutes at a specific temperature. The Au MBT PANI AChE PVAc biosensor was then evaluated in the 1 ml test solution with small aliquots of the substrate consisting of 0.01 M acetylthiocholine ATCh being added to the test solution followed by degassing. The maximum current .
