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Cathelicidins comprise a family of antimicrobial peptides sharing a highly conserved cathelin domain, which play a central role in the early innate host defense against infection. In the present study, we report three novel avian cathelicidin orthologs cloned from a constructed spleen cDNA library of Coturnix coturnix, using a nested-PCR-based cloning strategy. | IFEBS Journal Gene cloning expression and characterization of avian cathelicidin orthologs Cc-CATHs from Coturnix coturnix Feifei Feng1 2 z Chen Chen3 z Wenjuan Zhu2 Weiyu He1 Huijuan Guang2 Zheng Li2 Duo Wang1 Jingze Liu1 Ming Chen5 Yipeng Wang4 and Haining Yu1 2 1 College of Life Sciences Hebei Normal university Shijiazhuang Hebei China 2 Schoolof Life Science and Biotechnology Dalian University of Technology Dalian Liaoning China 3 College of BiologicalScience and Engineering Shaanxi University of Technology Hanzhong Shaanxi China 4 BiologicalResources Laboratory Yantai Institute of CoastalZone Research Chinese Academy of Sciences Yantai Shandong China 5 Department of Nephrology Teaching Hospitalof Chengdu University of TraditionalChinese Medical Chengdu China Keywords cathelicidin Coturnix coturnix expression molecular cloning structure and function Correspondence H. Yu or Y. Wang College of Life Sciences Hebei Normal university Shijiazhuang Hebei 050016 China BiologicalResources Laboratory Yantai Institute of CoastalZone Research Chinese Academy of Sciences Yantai Shandong 264003 China Fax 86 311 86268842 Tel 86 311 86268842 E-mail joannyu@live.cn wyp010@163.com These authors contributed equally to this work Received 7 November 2010 revised 20 February 2011 accepted 23 February 2011 doi 10.1111 j.1742-4658.2011.08080.x Cathelicidins comprise a family of antimicrobial peptides sharing a highly conserved cathelin domain which play a central role in the early innate host defense against infection. In the present study we report three novel avian cathelicidin orthologs cloned from a constructed spleen cDNA library of Coturnix coturnix using a nested-PCR-based cloning strategy. Three coding sequences containing ORFs of 447 465 and 456 bp encode three mature antimicrobial peptides named Cc-CATH1 2 and 3 of 26 32 and 29 amino acid residues respectively. Phylogenetic analysis indicated that precursors of Cc-CATHs are significantly conserved with known avian .
