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Green synthesis method is being increasingly used in the development of safe, stable, and eco-friendly nanostructures with biological resources. In this study, extracellular and intracellular synthesis of gold nanoparticles (AuNPs) was carried out using green algae Chlorella sorokiniana Shihira & R.W. Fresh algae were isolated and identified from Musaözü Pond located in the province of Eskişehir and then extraction process were performed. Optimization studies were studied using pH value, metal salt concentration, and time parameters for extracellular synthesis and using only time parameter for intrasellular synthesis. Since more controlled and optimum conditions can be achieved in the production of AuNPs by extracellular synthesis, these nanoparticles (NPs) were used for characterization and antifungal activity studies. | Turkish Journal of Biology Turk J Biol 2021 45 196-213 http journals.tubitak.gov.tr biology TÜBİTAK Research Article doi 10.3906 biy-2010-64 Synthesis of intracellular and extracellular gold nanoparticles with a green machine and its antifungal activity 1 2 2 3 Nurbanu GÜRSOY Betül YILMAZ ÖZTÜRK İlknur DAĞ 1 Eskişehir Osmangazi University Institute of Science Biotechnology and Biosafety Department Eskişehir Turkey 2 Eskişehir Osmangazi University Central Research Laboratory Application and Research Center Eskişehir Turkey 3 Vocational Health Services High School Eskisehir Osmangazi University Eskisehir Turkey Received 26.10.2020 Accepted Published Online 24.01.2021 Final Version 20.04.2021 Abstract Green synthesis method is being increasingly used in the development of safe stable and eco-friendly nanostructures with biological resources. In this study extracellular and intracellular synthesis of gold nanoparticles AuNPs was carried out using green algae Chlorella sorokiniana Shihira amp R.W. Fresh algae were isolated and identified from Musaözü Pond located in the province of Eskişehir and then extraction process were performed. Optimization studies were studied using pH value metal salt concentration and time parameters for extracellular synthesis and using only time parameter for intrasellular synthesis. Since more controlled and optimum conditions can be achieved in the production of AuNPs by extracellular synthesis these nanoparticles NPs were used for characterization and antifungal activity studies. Optical physical and chemical properties of synthesized NPs were characterized by UV visible spectrophotometer UV-Vis dynamic light scattering DLS Zetasizer X-Ray diffraction XRD Fourier transform ınfrared spectroscopy FTIR field emission scanning electron microscope FE-SEM ınductively coupled plasma mass spectrometer ICP-MS and transmission electron microscope TEM analysis. The optimum conditions for AuNPs synthesis were determined as 1 mM for HauCl4 .
