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In the present studies treated the seeds using gamma rays different does 10 kR to 100 kR each treatment using 50 seeds. The percentage of seed germination ranges from 0 per cent to 52 per cent with different does of gamma rays in butter bean variety KKL-1 as compared to 86 per cent control. The different in seed germination are statistically significant. The LD50 value was found at 10 kR for seed germination. The percentage of survival was 81 per cent in control while it are significant range from 0 per cent 100 kR to 48 per cent 10 kR of gamma rays in butter bean variety KKL-1. The LD50 value for seedling survival has to fixed at 10 kR. | Determination of lethal dose for gamma rays induced mutagenesis in butter bean Phaseolus lunatus L variety KKL-1