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Now a day’s nanotechnology has become one of the most promising approaches in pest management. Nanoparticles represent a new generation of environmental remediation technologies that provide cost–effective solution to some of the most challenging insect pests. S.litura (F.) (Lepidoptera: Noctuidae) is a major polyphagous pest of field crops and vegetables, causing severe damage to the plants in all phenological crop stages. The overall objective of this investigation was to look for new control strategy through evaluating the effect of Soya based Ag Nano -particles on the 2nd and 3rd instar larvae of S. litura under laboratory conditions. In the present study, we have reported the synthesis of silver green nanoparticles from soybean seed extract by Sunlight exposure method and its influence on S.litura. The synthesized soybean based silver nanoparticles were characterized by UV-Vis spectroscopy and particle size analyser (Nicomp). The result revealed that soybean based AgNo3 nanoparticles had a mean diameter of 87 nm (PSA).Nano Ag particles were the most effective compounds possessing insecticidal activity. Increased concentration and exposure period enhanced larval mortality. AgNPs caused characteristic symptoms of sluggishness, inactiveness, the larvae refusing to feed resulting in larval mortality. | Synthesis of green silver nanoparticles from soybean seed and its bioefficacy on Spodoptera litura (F.)