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The use of enzymes in a free form is very uneconomical because the enzymes generally cannot be recovered at the end of the reaction. These drawbacks can be overcome by immobilization of the enzyme thereby rendering it more stable and easy to recover and recycle. It is a very effective alternative for gripping the problems of instability, repetitive use and reduction in the cost of enzyme. The aim of this research was to obtain the optimum condition of the making of immobilized amylase beads using a different natural matrix. The result obtain that amylase producing isolate i.e. isolated from hot water spring and was precipitate with ammonium sulphate with specific activity of 45.29 IU/mg and 1.43 fold purification. The purified amylase was immobilized by entrapment method with 3% concentration of sodium alginate and agar with immobilization yield of 72.18% and 34.89% for isolate MW2 and 0.25% chitosan concentration gave 66.45% immobilization yield. Hence, we propose that, this method can be used to produce immobilized amylase which can be used in various areas such as diagnostics, food, medicine and cosmetics. | Immobilization of amylase by entrapment method in different natural matrix
