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The objective of this study was to detect antibiotic residues in sheep liver collected from Albaraka and Alsabaloga slaughterhouses at Karray locality in Khartoum state. A total of 28 sheep liver samples were taken to microbiology laboratory, microbial inhibition one plate of Bacillus subtilis in agar medium was used. The results revealed that 7 (25%) of sheep livers were positive to antibiotic, while 21 (75%) of them were negative. In conclusion the use of antibiotics in food producing animals' must be under control; it should be given at recommended dose with appropriate supervision. Adequate holding period or withdrawal period should be observed in all slaughtered animals following therapeutic use of antibiotics in treating sick animals, in addition to continuous training veterinarians to know the importance of withdrawal period in avoiding tissue residues. | Detection of antibiotic residues in sheep liver at Almoilih slaughter houses at Karrey locality, Khartoum state, Sudan