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To study the effect of substrate concentration on soil enzyme Urease in selected soils. Forty soil samples were assayed to measure the activity of the soil enzyme Urease among them four soil samples two Alfisols and two Vertisolssoils with high activity were selected for further study. Urease activities of the surface soils expressed as µg of NH4 + released g-1 soil h-1 ranged from 5.9 to 16.0 with an average value of 8.74.Soil enzyme Urease activity increased with an increase in substrate concentration in the beginning and almost reached a plateau at a substrate concentration of 30mM for all the four soils. With further increase in substrate concentration, minimal change in enzyme activity was observed. | Int.J.Curr.Microbiol.App.Sci 2020 9 3 1150-1158 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 3 2020 Journal homepage http www.ijcmas.com Original Research Article https doi.org 10.20546 ijcmas.2020.903.134 Effect of Substrate Concentration on Soil Enzyme Urease J. Aruna Kumari1 P.C.Rao2 G.Padmaja3 and M. Madhavi4 1 Department of Biochemistry College of Agriculture PJTSAU Rajendranagar Hyderabad Telangana India 2Dean of Agricuilture Retd. PJTSAU 3Department of SSAC College of Agriculture PJTSAU India 4ACRPP Weed control Rajendranagar PJTSAU India Corresponding author ABSTRACT Keywords Alfisols Eadie -Hofstee Transformation Hanes - Wolf Transformation Lineweaver - Burk Transformation Article Info Accepted 05 February 2020 Available Online 10 March 2020 To study the effect of substrate concentration on soil enzyme Urease in selected soils. Forty soil samples were assayed to measure the activity of the soil enzyme Urease among them four soil samples two Alfisols and two Vertisolssoils with high activity were selected for further study. Urease activities of the surface soils expressed as pg of NH4 released g-1 soil h-1 ranged from 5.9 to 16.0 with an average value of 8.74.Soil enzyme Urease activity increased with an increase in substrate concentration in the beginning and almost reached a plateau at a substrate concentration of 30mM for all the four soils. With further increase in substrate concentration minimal change in enzyme activity was observed. Characteristics of enzyme activities like maximum enzyme reaction velocity Vmax and Michaelis constant Km were determined using Michealis - Menten equation similar to those determined in homogenous system. The Km value range from 0.49mM to 0.60mM in Lineweaver - Burk Transformation and 0.50mM to 0.76mM in Hanes - Wolf Transformation and in case of Eadie - Hofstee Transformation the Km value range from 0.62mM to 0.78mM. Vertisols showed more km value
