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The effects of different culture media and temperature on mycelial growth and sclerotial formation of Macrophomina phaseolina (Tassi) Goid inciting agent of castor root rot were studied in vitro. Eight different solid and liquid media were tested for their effect on mycelial growth and sclerotial formation of test fungus. Among the solid media, potato dextrose agar (90.00 mm) and Richard's agar (87.71 cm) were the best for fungus growth as well as for sclerotial formation followed by Elliott's medium (80.08 cm). | Effect of media and temperature on the growth and sclerotial formation of Macrophomina phaseolina (Tassi) goid causing root rot of castor