TAILIEUCHUNG - Effects of cultivating conditions on the mycelial growth of Ganoderma lucidumin submerged flask culture

Effects of cultivating conditions on the mycelial growth of Ganoderma lucidumin submerged flask culture has 5 contents: introduction, materlals and methods, results and discussion, condusions. | Bioprocess Engineering 19 1998 233-236 Springer-Verlag 1998 Effects of cultivating conditions on the mycelial growth of Ganoderma lucidum in submerged flask cultures . Yang . Liau Abstract In this paper the effects of environmental conditions on the mycelial growth of Ganoderma ỉucidum were investigated in shake flask cultures. The optimal temperature and pH were found to be around 30-35 C and 4 respectively in a glucose-ammonium chloride medium. The maximum mycelial concentration reached to around 350 mg 100 ml. The formation of mycelial pellets and their ultra structure was demonstrated to be greatly affected by cultivating conditions. Increasing surface aeration would be beneficial for mycelial growth. However too high rotating speed in shake cultures had a detrimental effect on the formation of mycelial pellets and the optimum was found to be 100 rpm. 1 Introduction Ganoderma ỉucidum Fr. Karst Polyporaceae is a species of basidiomycetes which belongs to polyporacceae or Ganodermaceae of Aphyllophorales. Its fruiting body is called Reishi in Japanese and Lingzhi in China. In the regions of China Japan and Korea Lingzhi has been a popular folk or oriental medicine to cure various human diseases such as hepatitis hypertension hype-rcholesterlemia and gastric cancer. Recent studies on this fungus have demonstrated many interesting biological activities including antitumour and anti-inflammatory effects and cytotoxicity to hepatoma cells. These studies also suggested that the carcinostatic substance in Lingzhi is a polysaccharide b- 1- 3 -D-glucan 1 2 . This polysaccharide seems to show promise as a new type of carcinostatic agent which might be useful in immunotherapy. Lingzhi because of its perceived health benefits has gained wide popularity as a health food in both Japan and China. Reishi cultivation has prospered in Japan China Taiwan and Korea. In addition attempts are being made to obtain useful cellular materials or to produce effective substances .

• Nông nghiệp
• Microorganism and media
• Cultivation of microorganism
• The Analytical methods
• Effect of initial pH
• Effect of cultivating temperature
• Effect of surface aeration