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Đặc tính probiotic trong điều kiện in vitro của chủng nấm men Saccharomyces boulardii SB2 được phân lập từ quả rụng ở Vườn Quốc gia Cúc Phương, đã được đánh giá dựa vào các đặc điểm sinh học và khả năng chống chịu trong môi trường ruột mô phỏng. Trong nghiên cứu này, chủng nấm men được nghiên cứu về động học sinh trưởng cho sản xuất sinh khối cao ở quy mô bình lắc và trong thiết bị lên men 30 lít. | Xác định điều kiện nuôi thích hợp thu sinh khối của nấm men Probiotic Saccharomyces Boulardii SB2 ở quy mô bình lắc và 30 lít VNU Journal of Science: Natural Sciences and Technology, Vol. 35, No. 3 (2019) 47-55 Original Article Culture Condition for High Biomass Production of Probiotic Yeast Saccharomyces Boulardii SB2 in Shake Flask and 30 Littre Scale Dao Thi Luong , Ha Thi Hang, Duong Van Hop VNU Institute of Microbiology and Biotechnology, 144 Xuan Thuy, Hanoi, Vietnam Received 25 March 2019 Revised 09 April 2019; Accepted 21 July 2019 Abstract: Probiotic properties in vitroof the Saccharomyces boulardii SB2 yeast, was isolated from fruit at Cuc Phuong National Park were evaluated based on biological characteristics and tolerance in simulated intestinal conditions. In the present work, studies were carried out to improve cell growth kinetics to produce cell mass of this biotherapeutic yeast in shake flaskand 30-L bioreactor levels. In case of shake flask, the highest biomass was obtained under the culture conditions as YM medium, at 37oC, pH 6, inoculum concentration of 5 %, with shaking for 32 h; the best sources of carbon and nitrogen were found to be glucose and mixture of peptone, yeast extract, malt extract; cell dry weight and cell density reached 2.43 g L-1 and 109 CFU/ml repetitively. During 30-L bioreactor cultivation, the maximal cell mass in agitation speed of 200 rpm, aeration rate of 1.2 v v - 1 min-1; at 32 h of incubation, was 3.58 g L-1 in pH controlled culture and 3.08 g L-1 in uncontrolled pH. This is considered as the first step for cell mass production of this probiotic yeast in industrial scale Keywords: Saccharomyces boulardii SB2, probiotic, biomass, shake flask, 30-L bioreactor. __ Corresponding author. Email address: luongdt@vnu.edu.vn https://doi.org/10.25073/2588-1140/vnunst.4879 47 VNU Journal of Science: Natural Sciences and Technology, Vol. 35, No. 3
