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The highest variable and parsimony informative sites were observed in the trnL intron region, while the lowest sites were seen in the trnV intron. Less variable sites for the trnV intron region were expected since no foreign sequences could be included in the analysis. | Turkish Journal of Botany Turk J Bot (2016) 40: 472-479 © TÜBİTAK doi:10.3906/bot-1506-45 http://journals.tubitak.gov.tr/botany/ Research Article Phylogenetic relationships among native Oxytropis species in Turkey using the trnL intron, trnL-F IGS, and trnV intron cpDNA regions 1, 2 3 4 Ayten TEKPINAR *, Seher KARAMAN ERKUL , Zeki AYTAÇ , Zeki KAYA Department of Molecular Biology and Genetics, Yüzüncü Yıl University, Van, Turkey 2 Department of Biology, Aksaray University, Aksaray, Turkey 3 Department of Biology, Faculty of Science and Arts, Gazi University, Ankara, Turkey 4 Department of Biological Sciences, Middle East Technical University, Ankara, Turkey 1 Received: 25.06.2015 Accepted/Published Online: 11.03.2016 Final Version: 19.07.2016 Abstract: We tested the phylogenetic utility of three chloroplast DNA loci, i.e. the trnL(UAA) intron, trnL(UAA)-F(GAA) intergenic spacer (IGS), and trnV(UAC) intron, across thirteen native Oxytropis species. Our objective was to determine whether any of these chloroplast DNA markers could be beneficial to figure out phylogenetic relationships among Oxytropis species. To increase the interspecific sampling, nine sequences of the trnL intron and trnL-F regions were retrieved from GenBank and included in the analyses. No sequence of the trnV intron region was available in the database and so only sequences of the native species were used for the analyses. Phylogenies derived from maximum likelihood and maximum parsimony analyses indicated that the trnL intron and trnV intron regions provided better resolution for relationships among species with respect to the trnL-F region. The highest variable and parsimony informative sites were observed in the trnL intron region, while the lowest sites were seen in the trnV intron. Less variable sites for the trnV intron region were expected since no foreign sequences could be included in the analysis. Oxytropis lazica was phylogenetically separated from native species and .
