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This study is conducted to demonstrate the effect of moisture on Aflatoxins production in peanut. Peanut kernel with 6%, 8%, 10%, 12% and 14% moisture are injected with 103CFU/g of strain A. flavus TUHT115 then packaged in sealed PVC bag and kept at 25oC in 4 months. The mold growth and Aflatoxin (AF) production are checked every two weeks. | Journal of Science & Technology 126 (2018) 021-025 Effect of Moisture on Aflatoxins Production of Isolated Aspergillus Flavus from Vietnamese Peanut Le Thi Phuong Thao1*, Le Thi Hong Hao1, Nguyen Thanh Trung1, Ta Thi Yen1, Pham Xuan Da2 1 2 National Institute for Food Control, No. 65, Pham Than Duat, Cau Giay, Hanoi, Viet Nam National office in Southern Region, No. 31, Han Thuyen, district 1, Ho Chi Minh, Viet Nam Received: May 25, 2017; Accepted: May 25, 2018 Abstract Aflatoxins are known as toxic chemical compounds having serious effect on animal’s and human’s health. Aflatoxins are prevalent in various foods in Vietnam. This study is conducted to demonstrate the effect of moisture on Aflatoxins production in peanut. Peanut kernel with 6%, 8%, 10%, 12% and 14% moisture are injected with 103CFU/g of strain A. flavus TUHT115 then packaged in sealed PVC bag and kept at 25oC in 4 months. The mold growth and Aflatoxin (AF) production are checked every two weeks. As the results, peanut sample with 6% and 8% moisture are not contaminated with AF after 4 months study; the samples with moisture 10%, 12% and 14% are detected to be contaminated with AF after 2 weeks study and only AFB1 & AFB2 are detected in studied samples. AFB1 contaminated level is higher than maximum permissible level (8 g/kg) as regulated in QCVN 8-1:2011/BYT after 4th week and total AF is higher than maximum permissible level (15 g/kg) after 6th week. This study also revealed that peanut moisture content of level from 10% and more are accumulated AFB1 and total AF have linear relative with moisture and time. Keywords: peanut, peanut moisture, peanut preservative, Aflatoxin, A. flavus 1. Introduction* grains conditioned to an initial moisture content of 10.5 and 13.5% were inoculated with a known quantity (50:50) of A. flavus and A.parasiticus the study over a period of four weeks, the afflatoxin accumulation were quantified by HPLC. Thefindings have shown that, maize grains dried to moisture .
