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In this study, the antioxidant activity of ethanol extract of Salvia halophila Hedge (Lamiaceae) was evaluated by 2 antioxidant assays, including phosphomolybdenum reduction and free radical scavenging activity. | Turk J Biol 32 (2008) 265-270 © TÜBİTAK Determination of Antimicrobial and Antioxidant Activities of Turkish Endemic Salvia halophila Hedge Sevil ALBAYRAK1, Ahmet AKSOY1, Ergin HAMZAOĞLU2 1 Department of Biology, Art and Science Faculty, Erciyes University, 38039 Kayseri - TURKEY 2 Department of Biology, Art and Science Faculty, Bozok University, 66100 Yozgat - TURKEY Received: 22.01.2008 Abstract: In this study, the antioxidant activity of ethanol extract of Salvia halophila Hedge (Lamiaceae) was evaluated by 2 antioxidant assays, including phosphomolybdenum reduction and free radical scavenging activity. The ethanol extract showed free radical scavenging activity with IC50 of 67.73 μg/mL in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant activity of the extract was found to be 84.87 ± 0.7 mg ascorbic acid equivalent (AAE)/g extract in phosphomolybdenum assay. In the addition to the antioxidant activity of the extract, the total phenolic content was measured. Total phenolic content of ethanol extract was found to be 8.21 ± 0.0 mg gallic acid equivalent (GAE)/g extract. Antimicrobial activity of the extract was examined against 13 bacteria and 2 yeasts by agar diffusion method. The ethanol extract of S. halophila had no effect against the tested yeasts. The ethanol extract showed antibacterial activity against all of the tested Gram (+) bacteria except Listeria monocytogenes at 1%, 2.5%, 5%, and 10% concentrations. The ethanol extract had no effect on the entire tested Gram (-) bacteria except Aeromonas hydrophila and Pseudomonas aeruginosa. Key Words: Salvia halophila Hedge, antimicrobial and antioxidant activity, phenolic content, DPPH Türkiye’de Endemik Salvia halophila Hedge’nin Antimikrobiyal ve Antioksidan Aktivitesinin Belirlenmesi Özet: Bu çalışmada Salvia halophila Hedge (Lamiaceae)’nin etanollü özütlerinin antioksidan aktivitesi fosfomolibdenum redüksiyonu ve serbest radikal süpürücü aktivite olmak üzere 2 antioksidan analizi ile .