TAILIEUCHUNG - Determination of antimicrobial and antioxidant activities of Turkish endemic salvia halophila hedge

In this study, the antioxidant activity of ethanol extract of Salvia halophila Hedge (Lamiaceae) was evaluated by 2 antioxidant assays, including phosphomolybdenum reduction and free radical scavenging activity. | Turk J Biol 32 (2008) 265-270 © TÜBİTAK Determination of Antimicrobial and Antioxidant Activities of Turkish Endemic Salvia halophila Hedge Sevil ALBAYRAK1, Ahmet AKSOY1, Ergin HAMZAOĞLU2 1 Department of Biology, Art and Science Faculty, Erciyes University, 38039 Kayseri - TURKEY 2 Department of Biology, Art and Science Faculty, Bozok University, 66100 Yozgat - TURKEY Received: Abstract: In this study, the antioxidant activity of ethanol extract of Salvia halophila Hedge (Lamiaceae) was evaluated by 2 antioxidant assays, including phosphomolybdenum reduction and free radical scavenging activity. The ethanol extract showed free radical scavenging activity with IC50 of μg/mL in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant activity of the extract was found to be ± mg ascorbic acid equivalent (AAE)/g extract in phosphomolybdenum assay. In the addition to the antioxidant activity of the extract, the total phenolic content was measured. Total phenolic content of ethanol extract was found to be ± mg gallic acid equivalent (GAE)/g extract. Antimicrobial activity of the extract was examined against 13 bacteria and 2 yeasts by agar diffusion method. The ethanol extract of S. halophila had no effect against the tested yeasts. The ethanol extract showed antibacterial activity against all of the tested Gram (+) bacteria except Listeria monocytogenes at 1%, , 5%, and 10% concentrations. The ethanol extract had no effect on the entire tested Gram (-) bacteria except Aeromonas hydrophila and Pseudomonas aeruginosa. Key Words: Salvia halophila Hedge, antimicrobial and antioxidant activity, phenolic content, DPPH Türkiye’de Endemik Salvia halophila Hedge’nin Antimikrobiyal ve Antioksidan Aktivitesinin Belirlenmesi Özet: Bu çalışmada Salvia halophila Hedge (Lamiaceae)’nin etanollü özütlerinin antioksidan aktivitesi fosfomolibdenum redüksiyonu ve serbest radikal süpürücü aktivite olmak üzere 2 antioksidan analizi ile .

• Sinh học
• Salvia halophila Hedge
• Antimicrobial and antioxidant activity
• Phenolic content
• Including phosphomolybdenum
• Ascorbic acid equivalent
• Antioxidant potential of Turkish pepper
• Ascorbic acid
• Ferric reducing ability of plasma
• Trolox equivalent antioxidant capacity
• Total phenolic content