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The present work examined the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extract from Achillea biebersteinii Afan. (Asteraceae). The essential oil exhibited antimicrobial activity against 8 bacteria, 14 fungi and the yeast C. albicans, whereas methanolic extract remained inactive. | Turk J Biol 30 (2006) 65-73 © TÜB‹TAK Biological Activities of the Essential Oil and Methanol Extract of Achillea biebersteinii Afan. (Asteraceae) 1 1 2 3 4 5 Özlem BARIfi , Medine GÜLLÜCE , Fikrettin fiAH‹N , Hakan ÖZER , Hamdullah KILIÇ , Hakan ÖZKAN , 6 7 Münevver SÖKMEN , Tülin ÖZBEK 1 Department of Biology, Faculty of Arts and Science, Atatürk University, 25240 Erzurum - TURKEY 2 Department of Genetic and Bioengineering, Faculty of Engineering and Architecture, Kay›sdagi, Yeditepe University, 34755 ‹stanbul - TURKEY 3 Department of Crop Science, Faculty of Agriculture, Atatürk University, 25240 Erzurum - TURKEY 4 Department of Chemistry, Faculty of Art and Science, Atatürk University, 25240 Erzurum - TURKEY 5 The Ministry of Health of Turkey, Erzurum Regional Hygiene Institute, 25240 Erzurum - TURKEY 6 Department of Chemistry, Faculty of Arts and Science Cumhuriyet University, 58140 Sivas - TURKEY 7 Department of Biology, Institute of Natural and Applied Sciences, Atatürk University, 25240 Erzurum - TURKEY Received: 09.03.2006 Abstract: The present work examined the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extract from Achillea biebersteinii Afan. (Asteraceae). The essential oil exhibited antimicrobial activity against 8 bacteria, 14 fungi and the yeast C. albicans, whereas methanolic extract remained inactive. The antioxidative capacity of the samples was evaluated by using DPPH (2,2-diphenyl-1-picrylhydrazyl) and β-carotene/linoleic acid assays. In both assays, the extract showed better antioxidative capacity than the oil. The extract reduced the stable free radical DPPH with lower IC50 value (89.90 µg/ml) than the oil (8900 µg/ml). In the β-carotene/linoleic acid assay, the samples were not effectively able to inhibit the linoleic acid oxidation, exhibiting only 22.7% (the extract) and 16% (the essential oil) inhibitions at 2 mg/ml, far below than that of BHT (97.0%). Total .
