Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
For the first time, the detection of DNA sequences, related to HIV (found in AIDS patients), using Methylene Blue (MB) as the redox indicator was performed. The voltametric signals have been investigated on bared chitosan (CS) modified; probe immobilized (DNA single strand) and target-hybridized (DNA double strand) electrodes sequentially by means of Cyclic Voltammetry (CV) and Square Wave Voltammetry (SWV). The response of hybridization between the probe and perfectly complementary, double-base mismatch, single-base mismatch of analyzed targets were clearly identified. The response of CS modified electrode with non-complementary target sequence and the response of CS-free electrode (adsorption only) with complementary target were used as control references to confirm whether the detected signals correspond trully to the hybridization and respond selectively to the targets. | Journal of Chemistry Vol. 44 3 P. 377 - 381 2006 RAPID ELECTROCHEMICAL DETECTION OF SINGLE AND DOUBLE NUCLEOTIDE MISMATCHES MUTATIONS OF SEQUENCES FROM HIV SaMpLES Received 15 April 2005 TRAN DAI LAM1 BUI TIEN TRINH2 NGUyEN Duc CHIEN2 1Faculty of Chemical Technology Hanoi University of Technology HUT international Training Institute for Materials Science HUT SUMMARY For the first time the detection of DNA sequences related to HIV found in AIDS patients using Methylene Blue MB as the redox indicator was performed. The voltametric signals have been investigated on bared chitosan CS modified probe immobilized DNA single strand and target-hybridized DNA double strand electrodes sequentially by means of Cyclic Voltammetry CV and Square Wave Voltammetry SWV . The response of hybridization between the probe and perfectly complementary double-base mismatch single-base mismatch of analyzed targets were clearly identified. The response of CS modified electrode with non-complementary target sequence and the response of CS-free electrode adsorption only with complementary target were used as control references to confirm whether the detected signals correspond trully to the hybridization and respond selectively to the targets. I - INTRODUCTION In recent years there has been considerable interest in the development of DNA sensors due to their numerous applications such as the analysis of unknown or mutant genes diagnosis of infectious agents in various environments and detection of analytes drugs pollutants etc. Traditional methods for DNA detection based on the radioisotopic and fluorescent detection are labor and time consuming and are thus not well suited for routine and rapid medical analyses particularly for point-of-care tasks 1 2 . Among some new approaches for DNA detection electrochemical detection has many advantages such as reduction of the assay time simple protocol and therefore can be used for on-site monitoring. In this context oligonucleotides ODN a short .
