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The detection of specific DNA sequences provides the basis for detecting a wide variety of microbial and viral pathogens. Traditional methods for DNA sequencing, based on the radioisotopic and fluorescent detection, are labor and time consuming, and are, thus, not well suited for routine and rapid medical analyses, particularly for point-of-care tasks. Fast electrochemical detection of DNA sequences may greatly reduce the assay time, simplify its protocol and therefore can be used for on-site monitoring. | SHORT COMMUNICATIONS RAPID ELECTROCHEMICAL DETECTION OF SINGLE AND DOUBLE NUCLEOTIDE MISMATCHES OF SHORT SEQUENCES FROM HIV SAMPLES Received 16th-March 2005 TRAN DAI LAM1 BUI TIEN TRINH2 NGUyEN NGOC THINH1 NGUyEN Duc CHIEN2 1Faculty of Chemical Technology Hanoi University of Technology HUT international Training Institute for Materials Science HUT The detection of specific DNA sequences provides the basis for detecting a wide variety of microbial and viral pathogens. Traditional methods for DNA sequencing based on the radioisotopic and fluorescent detection are labor and time consuming and are thus not well suited for routine and rapid medical analyses particularly for point-of-care tasks. Fast electrochemical detection of DNA sequences may greatly reduce the assay time simplify its protocol and therefore can be used for on-site monitoring. For the first time we report the construction of electrochemical DNA biosensor the principle of which is based on the specific recognition of lH i N H. 2 2e Methylene Blue Electrochemical methods used in this study were CV Cyclic Voltammetry and SWV Square Wave Voltammetry . The discrimination of totally complementary HIV against different sequences of A20 A AAA AAA AAA AAA AAA AAA A served as non-complementary sequence 2M-HIV A GAA GaA gag AaA TGG GTG C as double mismatch and 1M-HIV A GAA GGA GAG AAA TGG GTG C as single mismatch was very clear while the decrease in current peaks on CV and SWV was observed for HIV sequence no changes were registered for non-complementary and mismatched sequences. immobilized DNA probe sequences with examined target ones. In this study the detection of short HIV sequences all sequences in 5 3 A GAA GGA GAG AgA TGG GTG C found in AIDS patients was carried out by using the chitosan CS modified glassy carbon GC electrodes immobilized with pGEM probe sequence CTC TCG CAC CCA TCT CTC TCC TTC TAG and redox indicator from methylene blue MB a well-known organic dye . The reduction of MB proceeds by the .
