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Molecular identification and genetic diversity within species of the genera Hanseniaspora and Kloeckera has many contents: introduction, yeast strain, isolation of DNA for PCR ass, RAPD-PCR analysi, PFGE karyotypin, PCR-RFLP analysis of rDN, karyotyping,. | FEMS Yeast Research ELSEVIER FEMS Yeast Research 1 2002 279-289 www.fems-microbiology.org Molecular identification and genetic diversity within species of the genera Hacceciacpora and Kloeckera Neza Cadez ab Peter Raspor a Arthur W.A.M. de Cock b Teun Boekhout b MaudyTh. Smith b a Biotechnical Faculty Department of Food Science and Technology University of Ljubljana Jamnikarjeva 101 1000 Ljubljana Slovenia b Centraalpareaevoor SoOimmelccltures Yeast Division P.O. Box 85167 3508 AD Urrecht Netherlands C.bure. .o ssch . . B Dor N era Received 24 April 2001 received in revised form 13 September 2001 accepted 27 September 2001 rrv-d y. dis- - 0 01 First published online 20 November 2001 Abstract Three molecular methods RAPD-PCR analysis electrophoretic karyotyping and RFLP of the PCR-amplified ITS regions ITS1 ITS2 andtOe intervcuing 5.8hrDNAi werestudiedforaccurateihentificatiyy oypTứgsendRơra aod h ơeứẲRraspeciesaswellasfns determining inteft end mtsasofciflc rolationshiptofeastramstruOstedfrom daffereyi somceD and or gedgraphrcahydirsmcs re-tons. Of thernehree methona PWRiRFLPanaiysire t tTSregiontwiShresSriationxnaymese íSer ynd F i m propaitdasa rapidideytificatiod m sUoh to nisarimiyaee unAnbieunostybetwesdallstx0eứwsii rs-sard sooc-co yndthasilsglenooeercospore-rotming apinplateyeaer c eO A-ơteaein ZzetíCyen .Electronnbretifkarybtaptyd producodcCromosomaivrofilnedywhichtherevnyrpeciys fouldbe divrUedtytofaurgrvups ohc rnra limiter karyaropts. Althouyo mont oftUc dOstonins ixamiyod exbibiteda tommontpeeter-rpecbìc pattem a differentdeghee of chroa osomal-lypvth doinniorhhismand aheriable mimbemX cdsdmosomatDNAfrxnmentsweteopsrrvrd withmspccier.C-oitetann-ysir ch thxcf AbmeORAPD-XnRfinxesotmts obtainedwi-h onelO-merxstreer Swomieaosatell-tepsimets andonemmisafeli-te nairnes OfneretrOcllleters whichwith afsweacepiionsete inaon emenl ipr the groups areartieareconuizen in DNADNA homolngy ftudies. U0a2Federation of EdsopeanMierabinlngsaalgoc-xt-es. Wubiishxd .
