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The male seahorse incubates its young in a manner resembling that of a mammalian pregnancy. After the female deposits her eggs into the male’s brood pouch they are fertilized and the embryos develop and grow for several weeks until they are able to withstand the external environmental conditions independently, at which point they are irreversibly released. Although the precise function of the brood pouch is not clear, it is probably related to pro-viding a suitable protective and osmotic environment for the young. . | ềFEBS Journal The male seahorse synthesizes and secretes a novel C-type lectin into the brood pouch during early pregnancy Philippa Melamed Yangkui Xue Jia Fe David Poon Qiang Wu Huangming Xie Julie Yeo Tet Wei John Foo and Hui Kheng Chua Department of BiologicalSciences National university of Singapore Singapore Keywords Hippocampus comes C-type lectin cDNA library male pregnancy Correspondence P. Melamed Department Biological Sciences NationalUniversity of Singapore 14 Science Drive 4 Singapore 117542 Fax 65 6872 2013 Tel 65 6874 1882 E-mail dbsmp@nus.edu.sg Received 23 November 2004 revised 26 December 2004 accepted 6 January 2005 doi 10.1111 j.1742-4658.2005.04556.x The male seahorse incubates its young in a manner resembling that of a mammalian pregnancy. After the female deposits her eggs into the male s brood pouch they are fertilized and the embryos develop and grow for several weeks until they are able to withstand the external environmental conditions independently at which point they are irreversibly released. Although the precise function of the brood pouch is not clear it is probably related to providing a suitable protective and osmotic environment for the young. The aim of this project was to construct and characterize a cDNA library made from the tissue lining the pouch in order to help understand the molecular mechanisms regulating its development and function. The library profile indicates expression of genes encoding proteins involved in metabolism and transport as well as structural proteins gene regulatory proteins and other proteins whose function is unknown. However a large portion of the library contained genes encoding C-type lectins CTLs of which three full-length proteins were identified and found to contain a signal peptide and a single C-lectin domain possessing all the conserved structural elements. We have produced recombinant protein for one of these and raised antisera we have shown using Western analysis and 2D electrophoresis that
