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Symposia Lectures Monday Monday, July 9, 2007 A2-L1 J. Diffley No abstract available. A2-L2 Molecular bioimaging of gene transcription P. Cramer Gene Center, Munich, GERMANY In the first half of the talk, the current state of our ongoing structure-function analysis of the mRNA transcription cycle will be discussed. The crystallographic structures of the complete 12-subunit RNA polymerase II in free form and with bound DNA and RNA have been determined as atomic models. The structure of the complete Pol II elongation complex bound by the transcript cleavage factor TFIIS explained how Pol II uses a single tunable active site for both RNA synthesis and RNA cleavage | Symposia Lectures Monday Monday July 9 2007 A2-L1 J. Diffley No abstract available. A2-L2 Molecular bioimaging of gene transcription P. Cramer Gene Center Munich GERMANY In the first half of the talk the current state of our ongoing structure-function analysis of the mRNA transcription cycle will be discussed. The crystallographic structures of the complete 12-subunit RNA polymerase II in free form and with bound DNA and RNA have been determined as atomic models. The structure of the complete Pol II elongation complex bound by the transcript cleavage factor TFIIS explained how Pol II uses a single tunable active site for both RNA synthesis and RNA cleavage. Three structures of complexes of CTD-interacting proteins provided insights into the coupling of transcription elongation to mRNA processing and histone methylation and into Pol II recycling. The first structure of a Pol II complex with an RNA inhibitor and the first atomic structures of subcomplexes of the mediator of transcriptional regulation provided insights into gene regulation. In the second half of the talk recently published and unpublished data will be presented that will show the directions for future investigations. A set of new structures of Pol II bound to damaged DNA together with complementary functional assays revealed an unexpected mechanism of lesion recognition during transcription-coupled DNA repair. Soon we will obtain new insights into the structure and function of the distantly related RNA polymerase I which produces ribosomal RNA. This part of the talk will highlight the complementary use of various structural X-ray EM NMR techniques and functional studies in vitro and in vivo. 18 Monday Symposia Lectures A2-L3 Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells Y. Watanabe University of Tokyo Tokyo JAPAN Centromeric protection of cohesin from separase-cleavage at meiosis I is a hallmark of reductional chromosome segregation an essential step to .
