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Recent studies suggest that diverse types of intronic and small non-coding RNAs play pivotal regulatory roles, often as a part of ribonucleoprotein (RNP) complexes, in a variety of cellular proces-ses such as transcription and translation. Thus, isolation and char-acterization of novel RNP complexes, such as micro RNPs, small nuclear RNPs, small nucleolar RNPs, and heteronuclear RNPs, are crucial to understand the biological systems. | Quantitative Biology Abstracts Quantitative Biology E1-1 Development of mass spectrometry-based analytical platform for small RNAs and ribonucleoprotein complexes T. Isobe1 2 Y. Yamauchi1 K. Kakiuchi1 H. Kaji1 M. Taoka1 and N. Takahashi2 3 1 Tokyo Metropolitan University Tokyo JAPAN 2CREST JST Tokyo JAPAN 3Tokyo University of Agriculture and Technology Tokyo JAPAN Recent studies suggest that diverse types of intronic and small non-coding RNAs play pivotal regulatory roles often as a part of ribonucleoprotein RNP complexes in a variety of cellular processes such as transcription and translation. Thus isolation and characterization of novel RNP complexes such as micro RNPs small nuclear RNPs small nucleolar RNPs and heteronuclear RNPs are crucial to understand the biological systems. At present the analysis of RNAs and RNP complexes depends mainly on the techniques in genomics and RNA biochemistry which include the process of reverse transcription from RNA to cDNA however they often suffers from the error of the reverse transcriptase reaction arising from both RNA secondary structures and posttran-scriptional base modifications which in turn is difficult to characterize by standard RNA analytical approaches. Mass spectrometry could offer a number of advantages for RNA analysis for its potential to provide efficient solutions to analysis of the sequence structure modification and composition of RNA in addition to mapping interactions between proteins and RNA. Here we describe our attempts to develop a mass spectrometry-based analytical platform for small RNAs and RNP complexes. Our approach is essentially based on the instrumentation for shotgun proteomics where the direct nanoflow LC is coupled with high resolution Q-TOF IT-FT hybrid mass spectrometer. E1-2 Functional proteomic screening of lipases using click chemistry methods M. Schicher I. Jesse A. Hermetter and R. Birner-Gruenberger Graz University of Technology Graz AUSTRIA Functional proteomics aims at .
