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Osteopontin(OPN) is an arginine-glycine-aspartate (RGD)-contain-ing adhesive glycoprotein that was first identified as a major sialo-protein in bone and subsequently found to be expressed in kidney, brain, macrophages, vascular smooth muscle cells and many cells of epithelial linings. In this study we examined the expression of OPN in MDCK cells in different cellular confluences. | Transport Machineries Abstracts Transport Machineries B1-1 Osteopontin expression in polarized MDCK cells N. Tascene1 and Z. S. Isguder2 IAnkara University Faculty of Veterinary Medicine Ankara TURKEY 2University of Veterinary Medicine Department of Physiological Chemistry Hannover GERMANY Osteopontin OPN is an arginine-glycine-aspartate RGD -contain-ing adhesive glycoprotein that was first identified as a major sialoprotein in bone and subsequently found to be expressed in kidney brain macrophages vascular smooth muscle cells and many cells of epithelial linings. In this study we examined the expression of OPN in MDCK cells in different cellular confluences. The cells were examined at very low density half confluence or complete confluence. OPN levels were investigated by western blotting and RT-PCR. An increase in OPN expression was observed due to the increasing confluency and subsequent initiation of polarization. Expression profiles of flotillin-2 in the same cells was used as a control since this protein is ubiquitously produced in MDCK cells and its expression rates are independent of confluence and or polarization. Intracellular distribution of OPN was also monitored by confocal microscopy on preparations immunolabeled with anti-OPN antibodies. Staining patterns have also confirmed increased OPN expression during confluency in MDCK cells. We conclude that the expression of OPN in polarized MDCK cells is induced by increasing confluency and polarization. Key words Osteopontin polarization MDCK cells. B1-2 Biogenesis of VGF secretory granules in epithelial thyroid cells F. Gentile1 A. Corteggio2 S. Avitabile2 G. Call1 and L. Nitsch2 1IEOS CNR Napoli ITALY 2University Federico II Napoli ITALY VGF is a granin that is sorted to a regulated and polarized pathway of secretion when expressed in FRT epithelial cells. We are investigating the role of aggregation and interaction with lipid rafts in VGF protein sorting. We demonstrated that a fraction of the .
