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Intracellular fatty acid-binding proteins (FABPs) are small members of the superfamily of lipid-binding proteins, which occur in invertebrates and ver-tebrates. Included in this superfamily are the cellular retinoic acid-binding proteins and retinol-binding proteins, which seem to be restricted to verte-brates. | iFEBS Journal Characterization of a hemocyte intracellular fatty acid-binding protein from crayfish Pacifastacus leniusculus and shrimp Penaeus monodon Irene Soderhall1 Amornrat Tangprasittipap1 2 3 HaiPeng Liu1 Kallaya Sritunyalucksana2 Poonsuk Prasertsan3 Pikul Jiravanichpaisal1 4 and Kenneth Soderhall1 1 Department of Comparative Physiology Evolutionary Biology Centre Uppsala University Sweden 2 CENTEX shrimp BIOTEC Faculty of Science MahidolUniversity Thailand 3 Department of IndustrialBiotechnology Faculty of Agro-Industry Prince of Songkla University Songkla Thailand 4 BIOTEC NationalScience and Technology Development Agency NSTDA Pathomthani Thailand Keywords all-trans retinoic acid crustaceans fatty acid-binding protein hemocyte retinoic acidbinding protein Correspondence I. Soderhall Department of Comparative Physiology Evolutionary Biology Centre Uppsala University Norbyvagen 18A Uppsala 75236 Sweden Fax 46 18 4716425 Tel 46 18 4712817 E-mail Irene.Soderhall@ebc.uu.se Website http www.fu.uu.se jamfys index.html Received 29 March 2006 revised 27 April 2006 accepted 2 May 2006 doi 10.1111 j.1742-4658.2006.05303.x Intracellular fatty acid-binding proteins FABPs are small members of the superfamily of lipid-binding proteins which occur in invertebrates and vertebrates. Included in this superfamily are the cellular retinoic acid-binding proteins and retinol-binding proteins which seem to be restricted to vertebrates. Here we report the cDNA cloning and characterization of two FABPs from hemocytes of the freshwater crayfish Pacifastacus leniusculus and the shrimp Penaeus monodon. In both these proteins the binding triad residues involved in interaction with ligand carboxylate groups are present. From the sequence and homology modeling the proteins are probably FABPs and not retinoic acid-binding proteins. The crayfish transcript plFABP was detected at high level in hemocytes hepatopancreas intestine and ovary and at low level in hematopoietic tissue and testis.
