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Cộng đồng vi khuẩn hoặc các uẩn còn được gọi là hệ thống màng sinh học có thể được chia thành văn phòng phẩm và các điện thoại di động. Những văn phòng phẩm là những bộ phim của vi sinh vật cổ điển thường là trên các bề mặt rắn. Những người di động đã được đặt tên với một loạt các thuật ngữ như tập hợp, tập hợp, flocs, tuyết, hoặc biofilms. | 6 Mapping Biopolymer Distributions in Microbial Communities John R. Lawrence Adam P Hitchcock Gary G. Leppard and Thomas R. Neu CONTENTS 6.1 Introduction. 122 6.2 Methodology. 123 6.2.1 Handling Flocs for Microscopic Examination. 123 6.2.2 Epifluorescence Microscopy. 124 6.2.3 CLSM and 2P-LSM. 124 6.2.3.1 CLSM Limitations. 125 6.2.3.2 2P-LSM Limitations. 125 6.2.4 Synchrotron Radiation Soft x-ray Imaging . 126 6.2.4.1 STXM Limitations. 127 6.3 Targets and Probes. 127 6.3.1 Polysaccharides . 127 6.3.1.1 General Probes. 127 6.3.1.2 Lectins. 128 6.3.1.3 Antibodies. 131 6.3.2 Proteins-Lipids. 131 6.3.3 Nucleic Acids. 131 6.3.4 Charge Hydrophobicity. 132 6.3.5 Permeability. 133 6.4 Examination of EPS Bound and Associated Molecules . 133 6.5 Digital Image Analyses. 134 6.5.1 Quantitative In Situ Lectin Analyses. 135 6.6 Deconvolution. 136 6.7 3D rendering . 136 6.8 Conclusions . 137 Acknowledgments. 137 References . 137 1-56670-615-7 05 0.00 1.50 2005 byCRC Press 121 Copyright 2005 by CRC Press 122 Flocculation in Natural and Engineered Environmental Systems 6.1 INTRODUCTION Microbial communities or aggregates also known as biofilm systems may be divided into stationary ones and mobile ones. Stationary ones are the classical microbial films usually on solid surfaces. Mobile ones have been named with a variety of terms such as assemblages aggregates flocs snow or mobile biofilms.1 The techniques described in the following chapter apply to both biofilms and flocs. Aquatic aggregates river lake marine technical may be very different in terms of size composition density and stability.2 Lotic aggregates are structurally very stable as they are exposed to a constant shear force resulting in relatively small aggregates 5 to 300 n-m whereas lake or marine snow may be very fragile and much larger millimeters to meters . Both environmental aggregates are colonized to a certain degree by prokaryotic and eukaryotic microorganisms bacteria algae fungi protozoa . The bacterial .
