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Termination of translation in eukaryotes is triggered by two polypeptide chain release factors, eukaryotic class 1 polypeptide chain release factor (eRF1) and eukaryotic class 2 polypeptide chain release factor 3. eRF1 is a three-domain protein that interacts with eukaryotic class 2 polypeptide chain release factor 3 via its C-terminal domain (C-domain). | NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor Al - nn . 1 1 1 1 2 Fl I Il3 l Alll 2 1 All II 24 Alexey B. Mantsyzov Elena V. Ivanova Berry Birdsall Elena Z. Alkalaeva Polina N. Kryuchkova Geoff Kelly5 Ludmila Y. Frolova2 and Vladimir I. Polshakov1 1 Center for Magnetic Tomography and Spectroscopy M. V. Lomonosov Moscow State University Russia 2 Engelhardt Institute of Molecular Biology Russian Academy of Sciences Moscow Russia 3 Division of Molecular Structure MRC NationalInstitute for MedicalResearch London UK 4 ChemicalDepartment M. V. Lomonosov Moscow State University Russia 5 MRC BiomedicalNMR Centre NIMR London UK Keywords human eukaryotic class 1 polypeptide chain release factor eRF1 NMR structure and dynamics stop codon recognition specificity termination of protein synthesis Correspondence V. I. Polshakov Center for Magnetic Tomography and Spectroscopy M. V. Lomonosov Moscow State University GSP-1 Moscow 119991 Russia Fax 7 495 9394210 Tel 7 495 9394882 E-mail vpolsha@mail.ru Database The 1H 15N and 13C chemical shifts have been deposited in the BioMagResBank database http www.bmrb.wisc.edu under the accession number BMRB-15366. The structural data and experimental restraints used in calculations have been submitted to the Protein Data Bank under the accession numbers 2KTV for the open conformer and 2KTU for the closed conformer Re-use of this article is permitted in accordance with the Terms and Conditions set out at http www3.interscience.wiley. com authorresources onlineopen.html Received 17 December 2009 revised 1 April 2010 accepted 8 April 2010 doi 10.1111 j.1742-4658.2010.07672.x Termination of translation in eukaryotes is triggered by two polypeptide chain release factors eukaryotic class 1 polypeptide chain release factor eRF1 and eukaryotic class 2 polypeptide chain release factor 3. eRF1 is a three-domain protein that interacts with eukaryotic class 2 polypeptide chain release