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By exchanging cytoplasmic proteins with exogenously added proteins, antibodies or cytosol that has been prepared from cells at distinct stages of the cell cycle or differentiation, or from disease states, we can modulate the intracellular environment and reconstitute various physiological phenomena in semi-intact cells. The semi-intact cell method was originally established by Dr. Simons’ group to study polarized vesicular trafficking in Madin-Darby canine kidney (MDCK) cells (Ikonen et al., 1995). We have refined the method by coupling it with GFP-visualization techniques and have established many types of assay for cell cycledependent changes in organelle morphology and membrane trafficking. Using our analytical system, we can manipulate intracellular conditions and. | DNA REPAIR Edited by Inna Kruman INTECHWEB.ORG DNA Repair Edited by Inna Kruman Published by InTech Janeza Trdine 9 51000 Rijeka Croatia Copyright 2011 InTech All chapters are Open Access distributed under the Creative Commons Attribution 3.0 license which permits to copy distribute transmit and adapt the work in any medium so long as the original work is properly cited. After this work has been published by InTech authors have the right to republish it in whole or part in any publication of which they are the author and to make other personal use of the work. Any republication referencing or personal use of the work must explicitly identify the original source. As for readers this license allows users to download copy and build upon published chapters even for commercial purposes as long as the author and publisher are properly credited which ensures maximum dissemination and a wider impact of our publications. Notice Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published chapters. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials instructions methods or ideas contained in the book. Publishing Process Manager Alenka Urbancic Technical Editor Teodora Smiljanic Cover Designer Jan Hyrat Image Copyright ZF 2011. Used under license from Shutterstock.com First published October 2011 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org DNA Repair Edited by Inna Kruman p. cm. ISBN 978-953-307-697-3 ikrrr iJ OPEN ACCESS INI Cvn PUBLISHER INTECH open free online editions of InTech Books and Journals can be found at .
