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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: The strength of the HIV-1 3' splice sites affects Rev function. | Retrovirology BioMed Central Open Access The strength of the HIV-1 3 splice sites affects Rev function Susanne Kammler 1 3 Marianne Otte1 4 Ilona Hauber2 Jorgen Kjems3 Joachim Hauber2 and Heiner Schaal 1 Address 1Institut fur Virologie Heinrich-Heine-Universitat Dusseldorf Universitatsstr. 1 Geb. 22.21 D-40225 Dusseldorf Germany 2Heinrich-Pette-Institute for Experimental Virology and Immunology Martinistrasse 52 D-20251 Hamburg Germany 3Department of Molecular Biology University of Aarhus C.F. Mollers Allé Bldg. 1130 DK-8000 Aarhus C Denmark and 4Institut fur Genetik Heinrich-Heine-Universitat Dusseldorf Universitatsstr. 1 Geb. 26.03 D-40225 Dusseldorf Germany Email Susanne Kammler - suk@mb.au.dk Marianne Otte - mahipp@web.de Ilona Hauber - ilona.hauber@hpi.uni-hamburg.de Jorgen Kjems - jk@mb.au.dk Joachim Hauber - joachim.hauber@hpi.uni-hamburg.de Heiner Schaal - schaal@uni-duesseldorf.de Corresponding author Published 04 December 2006 Received 18 September 2006 Accepted 04 December 2006 Retrovirology 2006 3 89 doi l0.ll86 l 742-4690-3-89 This article is available from http www.retrovirology.cOm content 3 1 89 2006 Kammler et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The HIV-1 Rev protein is a key component in the early to late switch in HIV-1 splicing from early intronless e.g. tot rev to late intron-containing Rev-dependent e.g. gag vif env transcripts. Previous results suggested that cis-acting sequences and inefficient 5 and 3 splice sites are a prerequisite for Rev function. However we and other groups have shown that two of the HIV-l 5 splice sites Dl and D4 are efficiently used in vitro and in vivo. Here we focus on the efficiency of the HIV-l 3 splice sites taking into consideration to
